NOVEL FLUORESCENCE QUENCHING PROBE FOR MEASURING NUCLEIC ACID

The present invention provides a QProbe that can be fluorescently labeled at a lower cost. A nucleic acid probe labeled with a fluorescent dye whose emission is reduced when the probe is hybridized with a target nucleic acid is provided. The probe is labeled with a fluorescent dye at its 5' ter...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	KURATA, Shinya, TAKADA, Katsumi
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a QProbe that can be fluorescently labeled at a lower cost. A nucleic acid probe labeled with a fluorescent dye whose emission is reduced when the probe is hybridized with a target nucleic acid is provided. The probe is labeled with a fluorescent dye at its 5' terminal site via an ssH amino linker as shown below or at its 3' terminal site via a CA amino linker as shown below. The base sequence of the nucleic acid probe is so designed that, when the probe is hybridized with a target nucleic acid, at least one G (guanine) exists in the base sequence of the target nucleic acid at a position 1 to 3 bases apart from the base at the terminal site where the probe and the target nucleic acid are hybridized with each other.