USING A BLUE-SHIFTED REFERENCE DYE IN AN OPTICAL GLUCOSE ASSAY

USING A BLUE-SHIFTED REFERENCE DYE IN AN OPTICAL GLUCOSE ASSAY

The invention is directed to a competitive glucose binding affinity assay comprising a glucose receptor (typically mannan binding lectin) labeled with an assay fluorophore and a modified glucose analog (typically dextran) labeled with a reference fluorophore. In certain embodiments, the glucose anal...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: AASMUL, Soren, SVENNING KRISTENSEN, Jesper
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC MACROMOLECULAR COMPOUNDS
PHYSICS
POLYSACCHARIDES
TESTING
THEIR PREPARATION OR CHEMICAL WORKING-UP
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Beschreibung
Zusammenfassung:The invention is directed to a competitive glucose binding affinity assay comprising a glucose receptor (typically mannan binding lectin) labeled with an assay fluorophore and a modified glucose analog (typically dextran) labeled with a reference fluorophore. In certain embodiments, the glucose analog is dextran and is coupled to both a reference fluorophore and a quencher dye (e.g. hexamethoxy crystalviolet-1). Optionally the reference fluorophore is blue shifted relative to the assay fluorophore.