METHOD FOR PROVIDING A DNA-ENCODED LIBRARY, DNA-ENCODED LIBRARY AND METHOD OF DECODING A DNA-ENCODED LIBRARY

A method for providing a DNA-encoding library, the DNA-encoding library and a method of decoding a DNA-encoded library are presented. Many different DNA molecules are synthesized which differ from each other by comprising different DNA barcode sequences, wherein each DNA barcode sequence comprises at least a first coding region DNA sequence comprising at least a first part, a second part and a third part, wherein the second part is located between the first and third part and the second part differs between all the DNA molecules by at least two nucleotides. Each of the many different DNA molecules is bonded to at least a specific substance forming different DNA-substance conjugates, wherein the DNA-substance conjugates differ from each other by the specific substance and by their DNA molecules, wherein the first part and the third part encode information regarding the second part of the first coding region and wherein a certain first part and/or a certain third part uniquely codes for a certain group of DNA-substance conjugates which is smaller than the group of all DNA-substance conjugates in the DNA-encoded library. The DNA-encoded library has the advantage that, for example after an enrichment experiment performed with the library, the library may be decoded in a faster and less expensive manner than known DNA-encoded libraries.