NEW TECHNIQUE FOR GENOMIC LARGE FRAGMENT DIRECT CLONING AND DNA MULTI-MOLECULAR ASSEMBLY

NEW TECHNIQUE FOR GENOMIC LARGE FRAGMENT DIRECT CLONING AND DNA MULTI-MOLECULAR ASSEMBLY

A method for homologous recombination, the method comprising: processing two or more target nucleic acid molecules by using a first exonuclease, and then performing homologous recombination by using the processed target nucleic acid molecules in the presence of a second exonuclease and an annealing...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: FU, Jun, ZHANG, Youming, WANG, Hailong, STEWART, Adrian Francis
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
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Beschreibung
Zusammenfassung:A method for homologous recombination, the method comprising: processing two or more target nucleic acid molecules by using a first exonuclease, and then performing homologous recombination by using the processed target nucleic acid molecules in the presence of a second exonuclease and an annealing protein, wherein at least one sequence homologous region is shared between the target nucleic acid molecules in which homologous recombination occurs. Further provided is a kit for use in said method.