PHYTASE YEAPPA MUTANTS HAVING IMPROVED GASTRIC PROTEIN RESISTANCE AND ACID RESISTANCE AND INCREASED CATALYTIC EFFICIENCY

The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162thsite of the sequence set forth in SEQ ID NO.1 with glycine or proli...

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Bibliographische Detailangaben
Hauptverfasser:	NIU, Canfang, LUO, Huiying, HUANG, Huoqing, YANG, Peilong, WANG, Yaru, LI, Yangyang, DU, Yongkai, YAO, Bin
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162thsite of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230thsite of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.