PASSAGING AND HARVESTING FORMULATION FOR SINGLE-CELL HUMAN PLURIPOTENT STEM CELLS

The field of the invention is cellular and molecular biology and stem cells. Specifically, the disclosure is directed to a formulation for harvesting and passaging single cell human pluripotent stem cells comprising: (i) 1 mM to about 30 mM sodium citrate; (ii) a salt comprising 10 mM to 170 mM KCl...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	FELLNER, Thomas, NIE, Ying, ROWLEY, Jonathan, Allen, WALSH, Patrick, AHMADIAN BAGHBADERANI, Behnam
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The field of the invention is cellular and molecular biology and stem cells. Specifically, the disclosure is directed to a formulation for harvesting and passaging single cell human pluripotent stem cells comprising: (i) 1 mM to about 30 mM sodium citrate; (ii) a salt comprising 10 mM to 170 mM KCl or NaCl; and (iii) Ca2+/Mg2+-free Dulbecco's phosphate buffered saline (DPBS), wherein said formulation has an osmolarity of about 100 mOsmol/liter to about 350 mOsmol/liter. The formulation can be used for serial passaging and dislodging of pluripotent stem cells attached to 2D tissue culture vessels or grown in 3D suspension culture (small scale and large scale bioreactors) or any other application where passaging in the form of single cell population of stem cells is needed.