DEVICE FOR MONITORING THE SPATIAL AND TEMPORAL DYNAMICS OF THROMBIN

The device for monitoring the spatial and temporal dynamics of thrombin that includes a temperature-controlled sealed chamber with a transparent window and a light trap, said chamber being filled with a fluid medium and designed to be capable of accommodating a cuvette containing a test sample of blood plasma, and into which a coagulation activating insert is placed, the latter having a substance which initiates the coagulation process applied to the butt end thereof, at least one means of illumination of the sample designed to be capable of receiving the light scattering signal from the sample and at least one first means of irradiation designed to be capable of exciting the fluorescence signal of a special marker that forms in the sample during the cleavage of a fluorogenic substrate previously added to the sample by one of the proteolytic enzymes of the coagulation system, a means of optical photo/ video registration of light scattering /irradiation from the sample, a means for adjustment of pressure in the temperature-controlled sealed chamber designed to be capable of maintaining a pressure inside the chamber exceeding the atmospheric one, at that the device includes at least one second means of irradiation of the sample designed to be capable of exciting a fluorescence signal of the said marker, at least one first means of irradiation that provides irradiation of the sample in a direction perpendicular to the cuvette plane, and at least one second means of irradiation that provides irradiation of the sample at an angle to the cuvette plane.