METHOD FOR PRODUCTION OF ISOPRENE RECOMBINANT MICROORGANISM, GENE CONSTRUCT, VECTOR AND APPLICATION THEREOF

The present disclosure relates to method for enhanced production of metabolite including but not limiting to isoprene and isoprenoid through chromosomal integration of genes belonging to MEP pathway. The disclosure further relates a host cell for the production of the said metabolite. The method of...

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Hauptverfasser: BALKISAN DHOOT, Shrikant, AHUJA, Manmeet, JOSHI, Harshvardhan, SUMAN VARAVADEKAR, Jayesh, PRAFULL SETHIA, Piyush, ISAR, Jasmine, PANKAJ VORA, Mansi, RANGASWAMY, Vidhya, AMBALAL JAIN, Dharmendra, SANJAY BADLE, Sneh, KRISHNA REDDY CHIGULLAREVU, Hari, SURESH RATHOD, Rachana
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present disclosure relates to method for enhanced production of metabolite including but not limiting to isoprene and isoprenoid through chromosomal integration of genes belonging to MEP pathway. The disclosure further relates a host cell for the production of the said metabolite. The method of the present disclosure bypasses the cumbersome method of plasmid application for the production of metabolite. The disclosure also relates to a gene construct comprising MEP genes and auxotrophic markers and a vector comprising the said gene construct.