METHOD FOR COUNTING WHITE BLOOD CELLS IN A SAMPLE

The invention relates to a method for determining the number of white blood cells in a sample. The method is based on an image, or hologram, of the sample, obtained by an image sensor when the sample is illuminated by a light source. The hologram is reconstructed holographically, such as to obtain a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	ALLIER, Cédric, ALI CHERIF, Anais, HERVE, Lionel, DRANCOURT (PR.), Michel, ISEBE, Damien, MOREL, Sophie
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CALCULATING CINEMATOGRAPHY COMPUTING COUNTING ELECTROGRAPHY HANDLING RECORD CARRIERS HOLOGRAPHIC PROCESSES OR APPARATUS HOLOGRAPHY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHOTOGRAPHY PHYSICS PRESENTATION OF DATA RECOGNITION OF DATA RECORD CARRIERS TESTING
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Beschreibung
Zusammenfassung:	The invention relates to a method for determining the number of white blood cells in a sample. The method is based on an image, or hologram, of the sample, obtained by an image sensor when the sample is illuminated by a light source. The hologram is reconstructed holographically, such as to obtain a complex image, known as the reference complex image, representing a light wave transmitted by the sample, in a reconstruction plane. A holographic propagation operator is applied to the reference complex image, such as to produce a plurality of so-called secondary complex images, forming a stack of images, from which a profile is determined describing the evolution of an optical characteristic of the light wave transmitted by the sample along the axis of propagation of said light wave. The presence of white blood cells and the number thereof are determined according to the aforementioned profiles.