METHOD FOR DETERMINING AFFINITY BETWEEN LIGANDS AND A TARGET

The invention relates to a method for determining the affinity between ligands and a target. According to said method, a mixture is prepared with a target and a sample formed from a mixture of ligands in controlled proportions so that the ligands having an affinity with the target bind thereto; the mixture is filtered so as to keep only the target-ligand complexes; the separation of the target-ligand complexes is provoked; the ligands and the targets are separated; the ligands are recovered by filtration in a treated solution and said treated solution is analysed to identify the presence of the ligands; the treated solution being analysed by relatively quantifying the ligands, the ligands present in a larger quantity having the greatest affinity with the target.