COMPOSITION AND METHOD FOR IMPROVING SENSITIVITY AND SPECIFICITY OF DETECTION OF NUCLEIC ACIDS USING DCAS9 PROTEIN AND GRNA BINDING TO TARGET NUCLEIC ACID SEQUENCE

The present invention relates to a composition and a method for improving sensitivity and specificity of detection of nucleic acids using a dCas9 protein. On the basis of a functional difference between Cas9 and dCas9, using dCas9 and gRNA, which binds to a target nucleic acid sequence, in amplifica...

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Bibliographische Detailangaben
Hauptverfasser:	KIM, Yong Sub, SHIN, Yong
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a composition and a method for improving sensitivity and specificity of detection of nucleic acids using a dCas9 protein. On the basis of a functional difference between Cas9 and dCas9, using dCas9 and gRNA, which binds to a target nucleic acid sequence, in amplification of nucleic acids including DNAs, RNAs, and the like increases efficiency in amplification of nucleic acids and thereby can ultimately improve sensitivity and specificity of a target diagnosis.In particular, using dCas9 and gRNA thereof in a biosensor almost doubles the efficiency in amplification of nucleic acids, increases four times the difference between a non-target and a target, and prevents nonspecific amplification of a non-target, thereby greatly increasing sensitivity and specificity. Thus, the composition can be very usefully utilized for improving sensitivity and specificity of detection of nucleic acids.