METHOD FOR PRODUCING MUTANT ENZYME, AND MUTANT ALCOHOL ACYLTRANSFERASE

It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the st...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	YU Fujio, MIZUNASHI Wataru
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	It is intended to provide an efficient method for expressing a protein of interest as an active soluble recombinant protein in a transformant. The present invention provides a method for producing an enzyme having improved activity per recombinant as compared with a reference form, comprising the steps of: (1) preparing recombinants each expressing a mutant having an amino acid sequence derived from the amino acid sequence of the reference form by the substitution of one or more cysteine residues by other amino acid residues; (2) selecting a plurality of mutants that exhibit 50% or more activity per recombinant relative to 100% activity of the reference form; and (3) expressing a mutant in which corresponding amino acid residues are substituted at two or more positions among the respective positions of the substituted amino acid residues of the mutants selected in step (2).