A METHOD FOR MEASUREMENT OF A COMPONENT IN CELLS

A method for the measurement of a component of interest in cells of a cellular population in a sample.The method comprising the steps of:a) obtaining a quantitative measurement of a marker present in cells of the cellular population in the sample;b) determining, with the aid of a computer, an approx...

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Bibliographische Detailangaben
Hauptverfasser:	PANGARKAR, Chinmay, WASSON, James, R, MOHAN, Karan
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS PHYSICS TESTING
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	A method for the measurement of a component of interest in cells of a cellular population in a sample.The method comprising the steps of:a) obtaining a quantitative measurement of a marker present in cells of the cellular population in the sample;b) determining, with the aid of a computer, an approximate amount of cells in the cellular population present in the sample based on the measurement of part a);c) adding an amount of a cell marker to the sample, where the amount of said cell marker added is based on the results of part b), and wherein the cell marker binds specifically to the component of interest in cells of the cellular population and is configured to be readily detectable;d) assaying cells in the sample for marker bound to the component of interest; ande) determining the amount of the component of interest in cells of the cellular population of the sample based on the amount of marker bound to the component of interest.