PROBE FOR NUCLEIC ACID ENRICHMENT AND CAPTURE, AND DESIGN METHOD THEREOF

Disclosed are a probe for nucleic acid enrichment and capture and a design method thereof. The probe consists of a sense strand probe and an antisense strand probe, wherein both the sense strand probe and the antisense strand probe are probes without an overlapping design, and both are 30-89 bases i...

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Bibliographische Detailangaben
Hauptverfasser:	SHI, Weijie, JI, Binfeng, RUAN, Li, LI, Xuchao, LIN, Qinghua, TANG, Zhenghua
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are a probe for nucleic acid enrichment and capture and a design method thereof. The probe consists of a sense strand probe and an antisense strand probe, wherein both the sense strand probe and the antisense strand probe are probes without an overlapping design, and both are 30-89 bases in length. The 3' or 5' of the probe is biotin-labeled and can bind to the avidin on the magnetic beads. The two-way probe can increase the specificity of the capture (i.e. reducing the capture of genomic DNA at non-target sites), can significantly increase the capture of the sample mutant DNA and can increase the original copy number of the captured sample nucleic acid.