SYNTHON FORMATION

This disclosure provides, among other things, a composition comprising: a 5' exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are als...

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Hauptverfasser: DAVIS, Theodore, B, SUN, Luo, GARDNER, Andrew, HSIEH, Pei-Chung, EVANS, Thomas, C., Jr
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Sprache:eng ; fre ; ger
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creator DAVIS, Theodore, B
SUN, Luo
GARDNER, Andrew
HSIEH, Pei-Chung
EVANS, Thomas, C., Jr
description This disclosure provides, among other things, a composition comprising: a 5' exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title SYNTHON FORMATION
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