SYNTHON FORMATION

This disclosure provides, among other things, a composition comprising: a 5' exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are als...

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Bibliographische Detailangaben
Hauptverfasser: DAVIS, Theodore, B, SUN, Luo, GARDNER, Andrew, HSIEH, Pei-Chung, EVANS, Thomas, C., Jr
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:This disclosure provides, among other things, a composition comprising: a 5' exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.