METHOD FOR QUANTIFYING AMINO ACID AND AMINO ACID QUANTIFICATION KIT

[Problem] To provide: a method for selectively and easily quantifying an amino acid to be measured (L-form or D-form amino acid) with high sensitivity using aminoacyl-tRNA synthetase (AARS); and an amino acid quantification kit. [Solution] The present invention pertains to: a method for quantifying...

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Bibliographische Detailangaben
Hauptverfasser: YAMADA, Kenta, NAKATSUKA, Tomoko, KIDA, Mikiko, AOKI, Hideyuki, KANEKO, Shoji, KUGIMIYA, Akimitsu
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:[Problem] To provide: a method for selectively and easily quantifying an amino acid to be measured (L-form or D-form amino acid) with high sensitivity using aminoacyl-tRNA synthetase (AARS); and an amino acid quantification kit. [Solution] The present invention pertains to: a method for quantifying an amino acid (L-form or D-form amino acid) in a sample by means of AARS, said method being characterized in that AARS and an amino acid are released from an aminoacyl-AMP AARS complex once formed and are used once again for forming an aminoacyl-AMP AARS complex so as to ultimately produce a reaction product such as pyrophoric acid to be measured in an amount that is greater in terms of the number of moles than the amino acid included in the sample; and an amino acid quantification kit for implementing said method.