METHOD FOR ENHANCED PROTEIN PRODUCTION

The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein ( e.g., the growth phase) in a perfusion cell culture to a high cell density ( i.e., at least above about 40×10 6 cells/mL) and then switching to a protein production...

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Bibliographische Detailangaben
Hauptverfasser:	ARUNAKUMARI, Alahari, GARCIA, Javier, DAI, Xiao-Ping, MARTEL, Richard
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein ( e.g., the growth phase) in a perfusion cell culture to a high cell density ( i.e., at least above about 40×10 6 cells/mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH ( i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.