METHOD FOR MEASURING TARGET DNA

The present invention provides a method for adequately analyzing a target DNA after genomic DNA cleavage reaction with a restriction enzyme. More specifically, the present invention provides a method for measuring a target DNA, including (1) cleaving a genomic DNA with a restriction enzyme in a solu...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ABE, Koichi, MATSUNO, Tatsuki
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The present invention provides a method for adequately analyzing a target DNA after genomic DNA cleavage reaction with a restriction enzyme. More specifically, the present invention provides a method for measuring a target DNA, including (1) cleaving a genomic DNA with a restriction enzyme in a solution to obtain a mixed solution containing (a) completely cleaved DNA fragments consisting of the target DNA, (b) incompletely cleaved DNA fragments comprising the target DNA, and (c) DNA fragments not comprising the target DNA; (2) removing the DNA fragments of (b) from said mixed solution to obtain a solution containing the DNA fragment of (a) and the DNA fragments of (c); and (3) analyzing the target DNA in the solution obtained in (2) .