METHOD FOR SEPARATING NUCLEIC ACID FROM SAMPLE COMPRISING NUCLEIC ACID, AND DEVICE THEREFOR

An object of the present invention is to provide a method for efficiently separating a nucleic acid from a specimen containing a nucleic acid without the use of specific equipment, and a device therefor. The method of the present invention uses a device comprising a treatment reagent-accommodating container 100 accommodating a treatment reagent for causing a nucleic acid to be released 101 and a nucleic acid-collecting member 200 comprising a nucleic acid adsorptive carrier 205, which is configured such that a cover 104 is attached to the treatment reagent-accommodating container 100, and when the treatment reagent-accommodating container 100 is connected to the nucleic acid-collecting member 200, a seal with the cover 104 is opened, thereby allowing a discharge opening 102 and a treatment reagent-supplying opening 204 to be communicated with each other, the method comprising: step 1 of accommodating a specimen in the nucleic acid-collecting member 200; step 2 of connecting the treatment reagent-accommodating container 100 to the nucleic acid-collecting member 200, thereby causing the treatment reagent-accommodating container 100 to be communicated with the nucleic acid-collecting member 200; step 3 of mixing the specimen and the treatment reagent to obtain a mixture 421, thereby causing a nucleic acid to be released; and step 4 of allowing the mixture 421 to pass through the carrier 205 so as to be discharged, thereby allowing the carrier 205 to adsorb the nucleic acid.