SYSTEM AND METHOD FOR MEASURING SULPHITE IN FOOD SAMPLES USING AN AMPEROMETRIC BIOSENSOR AND THE USE OF SAID BIOSENSOR

The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a...

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Bibliographische Detailangaben
Hauptverfasser:	ARANTZAMENDI EGIGUREN, Alai, GONZALEZ URQUIDI, Irune, JAUREGUIBEITIA CAYROLS, Arrate, MAZA DEL RIO, Sonia, SALLERES ALONSO, Sandra, GONZALEZ RIOJA, Roberto
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a system for measuring sulphite in food samples using a biosensor, a method for determining sulphite in food samples using said biosensor, and the use of said biosensor for measuring the sulphite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system, a working electrode, a counter electrode and a reference electrode. The bi-enzymatic system is composed of the human sulphite oxidase and peroxidase enzymes, and the immobilisation of the enzymes in the working electrode is carried out via simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the following phases: applying a sample to the amperometric biosensor; changing the electrical current upon coming into contact with the analyte to be detected; and amperometrically measuring said electrical current in solutions with continuous agitation, using a constant potential.