SYSTEM AND APPARATUS FOR BLIND DECONVOLUTION OF FLOW CYTOMETER PARTICLE EMISSION

Determining total particle emission of a flow cytometer particle by passing laser excitation energy into a generally perpendicular flow cytometer channel element through which a particle passes, the particle adapted to be irradiated and to emit a fluorescent emission responsive to the laser excitation energy, where the fluorescent emission results from a geometrical convolution of a geometrical form of the laser excitation energy and a geometric characteristic of a fluorescent emission of the particle, detecting any of the fluorescent emission, outputting an analog electrical emission, providing multiple time samples of the analog electrical emission, and processing the multiple time samples to calculate a total fluorescent emission of said particle, by determining the extent of said geometric convolution and fitting said time samples to a function of a same extent.