METHOD FOR DROPLET HANDLING, DETECTION AND SORTING

Methods, instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one aspect a method of preparing a droplet containing a single pair of two biological entities comprises providing a first pluralit...

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Bibliographische Detailangaben
Hauptverfasser:	CRAIG, Frank F, MARCHINGTON, Robert Frank, HOLMES, David, HAZELL, Michael Stuart, REHAK, Marian, PAWATE, Vinayaka C, SMITH, Clive A, DAY, Richard Francis, BENAZZI, Giuseppe, LIU, Xin, LI, Xin
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	Methods, instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one aspect a method of preparing a droplet containing a single pair of two biological entities comprises providing a first plurality of biological entities in a first fluid and providing a second plurality of biological entities in a second fluid and preparing a first droplet from said first fluid and preparing a second droplet from said second fluid. The method further comprises determining whether the first droplet contains a single entity of the first plurality of biological entities, and whether the second droplet contains a single entity of the second plurality of biological entities, and fusing the first droplet and the second droplet which have been determined to contain a said single entity, respectively.