AFUCOSYLATED PROTEIN, CELL EXPRESSING SAID PROTEIN AND ASSOCIATED METHODS

The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protei...

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Hauptverfasser:	MAITY, Sunit, DAKSHINAMURTHY, Pravin Kumar, MALIWALAVE, Amol, BHIMA RAO, Sahana, GHOSH, Maloy, HALAN, Vivek, UNNIKRISHNAN, Divya, KHAN, Amir, HAZARIKA, Jahnabi, BANGALORE MUNIRAJU, Yogendra Manjunath, NAIR, Karthika, RODRIGUES, Kavitha Iyer, CHATTERJEE, Sohang, PRASAD, Bhargav, CHAKRABARTY, Subhra Prakash, DUBEY, Ashvini Kumar, BHATTACHARJEE, Sanghamitra, KAMANAGOWDA, Veeresha, SRINIVASAN, Sankarnarayanan, NATARAJAN, Bairavabalakumar, SHARMA, Ankurina, THANIGAIVEL, Aswini, SHENOY, Bharath Ravindra, HORA, Anuradha, MUKUNDA, Pavithra, MURUGESAN, Sathyabalan
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.