HUMAN SEBOCYTE PRECURSOR CELLS, HUMAN SEBOCYTES AND IN VITRO METHODS FOR OBTAINING THE SAME FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (HIPSC)

One aspect of the invention relates to an in vitro method for obtaining hiPSC-derived sebocyte precursor cells comprising the steps of: a) culturing, under feeder layer-free conditions, human induced pluripotent stem cells (hiPSC) in a sebocyte basal medium complemented with a combination of (i) ret...

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Bibliographische Detailangaben
Hauptverfasser:	ONTENIENTE, Brigitte, MARUOTTI, Julien, BOBIER, Anaïs, Charlotte
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	One aspect of the invention relates to an in vitro method for obtaining hiPSC-derived sebocyte precursor cells comprising the steps of: a) culturing, under feeder layer-free conditions, human induced pluripotent stem cells (hiPSC) in a sebocyte basal medium complemented with a combination of (i) retinoic acid (RA), (ii) bone morphogenetic protein 4 (BMP4) and (iii) epidermal growth factor (EGF), during a period of time ranging from about 5 days to about 15 days, so as to generate multipotent skin precursor cells, b) culturing, under feeder layer-free conditions, the multipotent skin precursor cells obtained at step a) in a sebocyte differentiation basal medium, during a period of time ranging from about 5 days to about 15 days, so as to generate hiPSC-derived sebocyte precursor cells, and c) collecting the hiPSC-derived sebocyte precursor cells obtained at step b).