ISOTHERMAL NUCLEIC ACID AMPLIFICATION

An isothermal process for amplifying a double-stranded nucleic acid target molecule that relies on upstream and downstream primers and an oligonucleotide, wherein at least a portion of the oligonucleotide is complementary to a portion of the target sequence intervening the upstream and downstream pr...

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Bibliographische Detailangaben
1. Verfasser:	Hoser, Mark Jay
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	An isothermal process for amplifying a double-stranded nucleic acid target molecule that relies on upstream and downstream primers and an oligonucleotide, wherein at least a portion of the oligonucleotide is complementary to a portion of the target sequence intervening the upstream and downstream primers, wherein the upstream and downstream primers do not amplify the target molecule independently of the oligonucleotide and wherein the oligonucleotide facilitates the separation of the double-stranded nucleic acid target molecule thereby allowing the primers access to bind to complementary single-stranded DNA on the target molecule.