IMPROVED BINDING ASSAY

What has been developed is an improved method of determining the binding constants between two molecules that requires significantly fewer materials and potentially less time (in the case of a whole cell analysis less time to grow cell cultures as fewer cells are required in the same analysis) to undertake. The method involves utilizing an NSB measurement in preferably an n-curve analysis in order to determine the K d and/or R t without having to complete actual measurements to determine the lower knee of the curve(s) in the n-curve analysis. Preparing the experiment utilizing the additional samples allows an experiment represented in a binding curve having an upper and a lower knee to no longer be required to run through the lower knee to a point of completion.