PREPARATION OF STABILIZER-FREE GOLD SOLS AND THEIR CONFIGURATION INTO A GELATIN-COATED PROTEASE-INDICATING HOMOGENEOUS (ONE STEP) ASSAY

A method of detecting protease activity in a sample. The method includes contacting the sample with a composition containing (i) a dispersion of gold nanoparticles, whose surface is coated with a gelatin; and (ii) CaCl2. A color change of the composition, a turbidity change of the composition, or bo...

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Bibliographische Detailangaben
Hauptverfasser:	HAYRE, Paul N, KERSCHENSTEINER, Daniel, A
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of detecting protease activity in a sample. The method includes contacting the sample with a composition containing (i) a dispersion of gold nanoparticles, whose surface is coated with a gelatin; and (ii) CaCl2. A color change of the composition, a turbidity change of the composition, or both, upon the contacting of the sample with the composition, indicates the presence of protease activity in the sample. A kit adapted for performing the method. The kit includes (i) a vial containing a composition comprising CaCl2 and a dispersion of gold nanoparticles whose surface is coated with a gelatin; (ii) a cap configured for attachment to the vial, comprising a rubber septum; and (iii) a sampling device configured for collecting a fluid from a wound.