ANALYTE CONCENTRATION MEASUREMENT

A method of determining analyte concentration uses a redox reaction in an electrochemical cell that has at least two electrodes, one of which is a working electrode, at least one electrode being exposed to at least one redox mediator. The method uses at least one cycle of pulses, each cycle having at least a first potential and a second potential. The method comprises applying a first potential to initiate an accumulation phase that forces accumulation of a mediator concentration gradient at or close to the working electrode with a concentration that decreases towards the bulk solution, applying a second potential to initiate a measurement phase and deplete the established mediator concentration gradient at the working electrode, measuring current associated with the second potential of each cycle, and using the measured current to determine analyte concentration. In this way, the effects of diffusion interfering factors (DIF), especially haematocrit (Hct) can be mitigated.