STRAND-INVASION BASED DNA AMPLIFICATION METHOD

Methods for amplification of a target nucleic acid sequence comprising strand invasion are provided in which strand invasion occurs both at upstream and downstream regions of the target nucleic acid sequence. Further provided are kits and compositions suitable for use in such methods. The methods ma...

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Bibliographische Detailangaben
Hauptverfasser: EBOIGBODIN, Kevin, BRUMMER, Mirko
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:Methods for amplification of a target nucleic acid sequence comprising strand invasion are provided in which strand invasion occurs both at upstream and downstream regions of the target nucleic acid sequence. Further provided are kits and compositions suitable for use in such methods. The methods may comprise amplifying a target nucleic acid sequence comprising a region of unknown sequence, or determining the sequence of a target nucleic acid comprising a region of unknown sequence.