CHELATOR MODIFIED MAGNETIC SILICA NANOPARTICLES, THEIR USE AND PREPARATION

The present solution relates to magnetic probes/nanoprobes (NP) for the selective enrichment of proteins. Namely, the solution relates to magnetic nanoparticles, which surface is chemically modified with metal ion chelation groups. The use of these magnetic nanoparticles allows the separation of spe...

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Bibliographische Detailangaben
Hauptverfasser:	DANIEL DA SILVA, Ana Luísa, OLIVEIRA SILVA, Rui Pedro, PINHEIRO VITORINO, Rui Miguel
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BASIC ELECTRIC ELEMENTS CASTING CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY ELECTRICITY INDUCTANCES MAGNETS MAKING METALLIC POWDER MANUFACTURE OF ARTICLES FROM METALLIC POWDER METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL POWDER METALLURGY SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES SEPARATION THEIR RELEVANT APPARATUS TRANSFORMERS TRANSPORTING WORKING METALLIC POWDER
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Beschreibung
Zusammenfassung:	The present solution relates to magnetic probes/nanoprobes (NP) for the selective enrichment of proteins. Namely, the solution relates to magnetic nanoparticles, which surface is chemically modified with metal ion chelation groups. The use of these magnetic nanoparticles allows the separation of specific proteins from complex mixtures by means of a magnetic field, namely proteins containing metal ions in their composition, such as metalloproteases, and the recovery of metal binding proteins, such as histidine-tagged recombinant proteins. This solution describes a probe for select metalloproteins in a solution comprising at least an inorganic magnetic core particle comprising a paramagnetic, superparamagnetic or ferromagnetic material; wherein such particle is coated with a siliceous coating; wherein the siliceous coating further comprises a plurality of metal ion chelating moieties; wherein the size of the probe is less than 1100 nm. Furthermore, this disclosure also describes a method for producing said probe.