CELL CULTURE METHOD AND CELL CULTURE DEVICE

When cell culture is conducted by supplying a culture medium together with cells to a container 1, an upward movement step in which the container 1 is moved upward to a position higher than the initial position and a downward movement step in which the container 1 that has been moved upward is retur...

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Bibliographische Detailangaben
1. Verfasser:	HATA, Norihiko
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	When cell culture is conducted by supplying a culture medium together with cells to a container 1, an upward movement step in which the container 1 is moved upward to a position higher than the initial position and a downward movement step in which the container 1 that has been moved upward is returned to the initial position, and vibration is applied to the container 1 that has been returned to the original positon are repeated, whereby a content liquid in the container 1 is stirred. As a result, a good culture environment is maintained by conducting stirring such that distribution of cells and concentration of components of a culture medium in a content liquid become uniform, while reducing damage on cells by shear stress, and adherence of cells to the inner wall or excessive agglomeration is suppressed by stirring, whereby proliferation of cells can be promoted.