A COMBINATION OF A REACTION APPARATUS AND AN OPTICAL INSTRUMENT MONITORING DNA POLYMERASE CHAIN REACTIONS

An optical instrument (A) for monitoring polymerase chain reaction replication of DNA may include a thermal cycler block (1c) for holding a plurality of vials (1b), each containing a suspension of ingredients that include a fluorescent primary dye. The instrument may include a light source (11) for...

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Bibliographische Detailangaben
Hauptverfasser:	YOUNG, EUGENE F, LAKATOS, EDWARD J, GAMBINI, MICHAEL R, ATWOOD, JOHN G, CERRONE, ANTHONY L
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	An optical instrument (A) for monitoring polymerase chain reaction replication of DNA may include a thermal cycler block (1c) for holding a plurality of vials (1b), each containing a suspension of ingredients that include a fluorescent primary dye. The instrument may include a light source (11) for emitting a source beam, a first means (7) disposed to be receptive of the source beam, a primary focusing means (3) disposed to focus the excitation beam simultaneously into a plurality of suspensions and being receptive of and passing emission beams, a second means (8) disposed to be receptive of emission beams, an emission focusing means (10) for focusing emission beams, a detector, and a processing means for computing concentration of DNA. The optical system being arranged without a beam splitter (6) but with one or more folding mirrors (5), and with excitation and emission beams being on slightly different optical paths angularly.