REAL TIME QUANTITATIVE AND QUALITATIVE ANALYSIS METHOD FOR BIOSUBSTANCE

Disclosed is a method of quantitatively and qualitatively analyzing a biomaterial in real-time. More particularly, disclosed is a method allowing real-time quantitative and qualitative analyses of a biomaterial by integrally performing real-time Polymerase Chain Reaction (PCR) and DNA microarray in the same space. According to the method of quantitatively and qualitatively analyzing a biomaterial in real-time of the present invention, inconvenience and low economic feasibility of conventional technologies requiring several to dozens of reaction containers (tubes) depending upon gene types can be addressed. In addition, the method can address a disadvantage of DNA microarrays, application of which to quantitatively analysis is difficult. In particular, the method of quantitatively and qualitatively analyzing a biomaterial in real-time according to the present invention enables the quantitative and qualitative analyses to be possible without opening or washing the container by obtaining signals of a DNA chip in a reaction container in real-time, and quantitative analysis and gene-type examination to be economically, reliably carried out at the same time in a variety of molecular diagnosis fields such as drug resistance examination, somatic mutation examination, and single nucleotide polymorphism examination as well as infectious disease examination. Therefore, the method of quantitatively and qualitatively analyzing a biomaterial in real-time according to the present invention can be easily used in all molecular diagnosis fields regardless of analysis type, gene type, or examination type.