ABSOLUTE QUANTITATION OF PROTEINS AND PROTEIN MODIFICATIONS BY MASS SPECTROMETRY WITH MULTIPLEXED INTERNAL STANDARDS

ABSOLUTE QUANTITATION OF PROTEINS AND PROTEIN MODIFICATIONS BY MASS SPECTROMETRY WITH MULTIPLEXED INTERNAL STANDARDS

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obt...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: PATEL, BHAVINKUMAR, HERMANSON, GREG, LOUETTE, JOEL, BOMGARDEN, RYAN, DANIEL, ROGERS, JOHN, CHARLES
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BASIC ELECTRIC ELEMENTS
ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
ELECTRICITY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.