Device and method for large volume transfection

The invention relates to a device 1 for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber 6 which comprises at least two segments, each segment comprising at least one electrode 4, 5, wherein at...

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Bibliographische Detailangaben
Hauptverfasser:	Gleissner, Timo, Hermsmeier, Sven, Altrogge, Ludger, Heinze, Andreas
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ELECTROTHERAPY ENZYMOLOGY HUMAN NECESSITIES HYGIENE MAGNETOTHERAPY MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RADIATION THERAPY SPIRITS ULTRASOUND THERAPY VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a device 1 for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber 6 which comprises at least two segments, each segment comprising at least one electrode 4, 5, wherein at least one port 7, 8 is disposed at one end 9 of the chamber 6 and at least one further port 10, 11 is disposed at the opposite end 12 of the chamber 6. According to the invention each segment is provided with at least one first electrode 4 and at least one second electrode 5, wherein the second electrode 5 is a common electrode of at least two segments. In order to avoid arcing and/or undesired heating of the suspension, voltage pulses can be applied to different segments of the chamber sequentially.