METHOD FOR SYNTHESIZING GENE LIBRARY USING CODON COMBINATION AND MUTAGENESIS

Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo. In addition, the present disclosure provides a method of simultaneously synthesizing and analyzing a gene library in which protein sequences are converted to some similar protein sequences in the same manner, and a method of identifying what effect a change in a partial protein sequence of the expressed gene has influenced on the function of the corresponding gene.