DETECTION METHOD, MICROARRAY ANALYSIS METHOD AND FLUORESCENCE READING DEVICE

A detecting method, a microarray analyzing method, and a fluorescence reading device, which enable acquirement of an image, from which a height difference of a substrate is detectable, are provided. In a detecting method of irradiating a substrate having a concave and convex shape with laser light collected by a lens, acquiring, as image data, received light intensity of reflected light and/or scattered light from the substrate, and detecting a height difference of the concave and convex shape, a light irradiation surface of the substrate is arranged at a position closer to the lens than a focal position of the lens is, reflected light and/or scattered light from the light irradiation surface is received as detected light, and a height difference of the substrate is detected based on a change in intensity of the received light.