MULTIPLEXING AND QUANTIFICATION IN PCR WITH REDUCED HARDWARE AND REQUIREMENTS

MULTIPLEXING AND QUANTIFICATION IN PCR WITH REDUCED HARDWARE AND REQUIREMENTS

Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgradin...

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Bibliographische Detailangaben
Hauptverfasser: SCHERER, Axel, SHAH, Sheel, M, MADERO, Xiomara, L, GARCIA, Erika, F, MALIK, Imran, R
Format: Patent
Sprache:eng ; fre ; ger
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BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
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Zusammenfassung:Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.