Method for the isolation of glycoprotein-rich fungal extract and its use in anti-ageing cosmetic formulations

The present invention relates to isolation of glycoprotein-rich fractions from strain of soil fungus Penicillium lanoso-viride strain 8D and use of them as active ingredients in anti-ageing cosmetic products. The object of the invention is the production of two types of glycoprotein fractions with different degree of purification. Enriched fraction is isolated by stepwise ammonium sulphate precipitation. To produce purified glycoprotein fraction enrichment is followed by gel filtration on Sephadex G-200 or G-150. Use of both glycoprotein fractions in skin regenerating cosmetic compositions is proposed - glycoprotein enriched fraction is used at concentration 5-15 mg/ml, purified fraction at concentration 5-10 µg/ml final compositions.