GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS

GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS

The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3- -D-glucan synthase-activity, and/or (ii) a polypeptide having 1,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HAEFNER, Stefan, HEROLD, Andrea, GRANSTRÖM, Mari, BROCKMANN, Beata, SCHMIDT, Julia, Kristiane, FLECK, Christian, SCHRÖDER, Hartwig, ZELDER, Oskar
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3- -D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3- -D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3- -D-glucan synthase-activity or the use of such a polypeptide for producing -glucans. Furthermore, the present invention relates to methods for producing -glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3- -D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3- -D-glucan synthase-activity into a microorganism being able to synthesize -glucans.