SUPERCOILED MINIVECTORS AS A TOOL FOR DNA REPAIR, ALTERATION AND REPLACEMENT

SUPERCOILED MINIVECTORS AS A TOOL FOR DNA REPAIR, ALTERATION AND REPLACEMENT

In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology-...

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Bibliographische Detailangaben
Hauptverfasser: MAIZEL, NANCY, CATANESE, JR., DANIEL, JAMES, FOGG, JONATHAN, ZECHIEDRICH, E,LYNN, BAKKALBASI, EROL, HUMBERT, OLIVIER
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Beschreibung
Zusammenfassung:In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology-directed repair, alteration, or replacement of the targeted DNA sequence within a cell in vivo or in vitro, where the MiniVector lacks both a bacterial origin of replication and an antibiotic selection gene, and wherein the Mini Vector has a size up to about 2,500 base pairs.