METHOD FOR DETECTING TARGET NUCLEIC ACID USING MOLECULAR BEACON-TYPE PROBE

The present invention provides a detection system using a nucleic acid probe which is commonly used in a measurement system using a recombinase (e.g., nucleic acid amplification reaction). Specifically, the present invention provides a method for detecting a target nucleic acid, comprising measuring...

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1. Verfasser: MATSUNO, TATSUKI
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention provides a detection system using a nucleic acid probe which is commonly used in a measurement system using a recombinase (e.g., nucleic acid amplification reaction). Specifically, the present invention provides a method for detecting a target nucleic acid, comprising measuring the target nucleic acid using a cleavage enzyme, a recombinase, and a probe having characteristics of the following (a) to (c): (a) being a single-stranded nucleic acid molecule comprising a pair of regions capable of complementarily binding to each other, and a region capable of complementarily binding to the target nucleic acid; (b) comprising a site to be cleaved by the cleavage enzyme; and (c) having a terminal label.