METHOD FOR CORRECTING DETECTION SIGNAL IN ISOTHERMAL NUCLEIC ACID AMPLIFICATION REACTION
The present invention provides a method for stably correcting a detection signal in an isothermal nucleic acid amplification reaction, particularly an isothermal nucleic acid amplification reaction performed at low temperature. Specifically, the present invention provides a method for detecting a ta...
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Format: | Patent |
Sprache: | eng ; fre ; ger |
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Zusammenfassung: | The present invention provides a method for stably correcting a detection signal in an isothermal nucleic acid amplification reaction, particularly an isothermal nucleic acid amplification reaction performed at low temperature. Specifically, the present invention provides a method for detecting a target nucleic acid in an isothermal nucleic acid amplification reaction, comprising the following (1) and (2):
(1) subjecting a nucleic acid sample to an amplification reaction of a target nucleic acid under an isothermal condition in the presence of a nucleic acid probe for correction and a nucleic acid probe for detection of the target nucleic acid; and
(2) correcting a detection signal due to the nucleic acid probe for detection with a detection signal due to the nucleic acid probe for correction in the amplification reaction. |
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