METHOD FOR IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM

Improved separation of a hydrophobic organic solution from an aqueous culture medium, comprises preparing an aqueous culture medium comprising a metabolically active cell, contacting the aqueous culture medium with a hydrophobic organic solution, and separating the hydrophobic organic solution from...

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Bibliographische Detailangaben
Hauptverfasser:	HAAS, Thomas, HAFKEMEYER, Sabine, SCHAFFER, Steffen, CORTHALS, Jasmin, HENNEMANN, Hans-Georg, WESSEL, Mirja, PÖTTER, Markus, ERHARDT, Frank, BERSE, Katharina
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Improved separation of a hydrophobic organic solution from an aqueous culture medium, comprises preparing an aqueous culture medium comprising a metabolically active cell, contacting the aqueous culture medium with a hydrophobic organic solution, and separating the hydrophobic organic solution from the aqueous culture medium, where a decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell, and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids. Independent claims are included for: (1) use of metabolically active cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, where the enzyme is configured to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active cell; (2) use of knockout of an enzyme that catalyses one reaction of the beta -oxidation of fatty acids, as part of a genetic make-up of the metabolically active to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active cell; (3) a cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, and a recombinant monooxygenase, preferably alkane hydroxylase; and (4) a reaction mixture, comprising an aqueous solution comprising the metabolically active cell having decreased activity, and a hydrophobic organic solution, where the decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids.