METHOD FOR CULTURING PLURIPOTENCY-MAINTAINED SINGLY DISPERSED CELLS BY MEANS OF LAMINAR FLOW

METHOD FOR CULTURING PLURIPOTENCY-MAINTAINED SINGLY DISPERSED CELLS BY MEANS OF LAMINAR FLOW

The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspec...

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Bibliographische Detailangaben
Hauptverfasser: TADA, Takashi, MATSUMURA, Taku, TATSUMI, Kazuya, YOKOKAWA, Ryuji, OKITSU, Teru, KOTERA, Hidetoshi, NODA, Yuichiro, OKONOGI, Atsuhito, OSUMI, Takashi, NAKANISHI, Naoyuki, NAGATA, Shogo
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The one aspect of the present invention aims to provide a novel cloning method for human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), which method is based on culture of dispersed single cells without addition of an apoptotic agent. More specifically, the one aspect of the present invention aims to provide a cloning method for hESCs and hiPSCs based on culture of dispersed single cells utilizing shear stress. The above problem is solved by providing a method for culturing dispersed single pluripotent cells, wherein the dispersed single cells are cultured under laminar flow conditions.