METHOD AND ANALYTICAL KIT FOR SIMULTANEOUS QUANTIFICATION OF B-COMPLEX VITAMIN CONTENT IN FOOD

The present invention relates to an analytical vitamin analysis kit, the method of its preparation, and the method of its application. The given analytical kit can be used for the simultaneous determination of the quantity of five B-complex vitamins (B l, B2, B3, B5 and B6) and individual vitamers in drugs, food, food- and bio-supplements, feed, and other biological samples using LC-MS isotope internal standard assay. The analytical kit is based on the use of sample vials containing lyophilized or dried isotope labelled internal standards of the B-complex vitamers with a mix of hydrolytic enzymes, used to convert vitamers and cofactors into the same chemical forms as the used isotope labelled internal standards, and for their following quantification by means of LC-MS or LC-MS/MS.