Recombinant deamidated gliadin antigen

The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes...

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Bibliographische Detailangaben
Hauptverfasser: SHAN, DAMING, BRUEHL, RICHARD, YANG, XIAOYUN, WATKINS, MICHAEL I, MARR, GREGORY A, COLEMAN, PATRICK F
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.