Charge-switch nucleotides based method for sequencing of nucleic acids

The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labelled NPs that exhibit charge-switching behaviour, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are de...

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Bibliographische Detailangaben
Hauptverfasser:	REYNOLDS, BAMBI, L, DRANEY, DAN, WILLIAMS, JOHN, G. K, BASHFORD, GREGORY, R, SHEAFF, PAMELA, NARAYANAN, NARA, CHEN, JIYAN
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS THEREFOR BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY GENERAL METHODS OF ORGANIC CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labelled NPs that exhibit charge-switching behaviour, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electricity sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.