Method for improved separation of a hydrophobic organic solution from an aqueous culture medium

Improved separation of a hydrophobic organic solution from an aqueous culture medium, comprises preparing an aqueous culture medium comprising a metabolically active cell, contacting the aqueous culture medium with a hydrophobic organic solution, and separating the hydrophobic organic solution from the aqueous culture medium, where a decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell, and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids. Independent claims are included for: (1) use of metabolically active cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, where the enzyme is configured to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active cell; (2) use of knockout of an enzyme that catalyses one reaction of the beta -oxidation of fatty acids, as part of a genetic make-up of the metabolically active to improve the separation of the hydrophobic organic solvent from the aqueous culture medium comprising the metabolically active cell; (3) a cell, comprising an enzyme of the beta -oxidation of fatty acids having a decreased activity, compared to a wild-type of the cell, and a recombinant monooxygenase, preferably alkane hydroxylase; and (4) a reaction mixture, comprising an aqueous solution comprising the metabolically active cell having decreased activity, and a hydrophobic organic solution, where the decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell, and the decreased activity is of at least of one enzyme that catalyses one reaction of beta -oxidation of fatty acids. Die vorliegende Anmeldung betrifft ein Verfahren zur verbesserten Abtrennung einer hydrophoben organischen Lösung von einem wässrigen Kulturmedium umfassend die Schritte, Bereitstellen eines wässrigen Kulturmediums umfassend eine stoffwechselaktive Zelle, Kontaktieren des wässrigen Kulturmediums mit einer hydrophoben organischen Lösung, Abtrennen der hydrophoben organischen Lösung von dem wässrigen Kulturmedium, wobei die Zelle eine ihrem Wildtyp gegenüber verringerte Aktivität wenigstens eines Enzyms aufweist, das eine der Reaktionen der ²-Oxidation von Fettsäuren katalysiert. Weiterhin betrifft die Erfindung die Verwendung einer stoffwechselaktiven Zelle, die eine ihrem Wildt